| A decade of liver organoids: Advancements in disease modeling |
| Yue Liu1,2, Jian-Ying Sheng1, Chun-Fang Yang1, Junjun Ding2,3, Yun-Shen Chan1 |
1Guangzhou Laboratory, No. 9 XingDaoHuanBei Road, Guangzhou International Bio Island, Guangzhou 510005, Guangdong Province, China
2Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, Guangdong, China
3Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, Guangdong, China |
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Received: November 29, 2022 Revised: January 13, 2023 Accepted: February 28, 2023
*Yue Liu, Jian-Ying Sheng and Chun-Fang Yang contributed equally to this work. |
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| ABSTRACT |
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Liver organoids are 3D cellular models of tissue in which cells interact to form unique structures in culture. Since their inception, liver organoids with various cellular compositions, structural features, and functional properties have been described over the past ten years. Methods to create these advanced human cell models range from simple tissue culture techniques to complex bioengineering approaches. These liver organoid culture platforms have been utilized in various liver research fields, including the modeling of liver diseases to regenerative therapy. This review will discuss how liver organoids are used to model diseases, including hereditary liver diseases, primary liver cancer, viral hepatitis, and nonalcoholic fatty liver disease. Specifically, we will focus on studies that utilized two widely adopted approaches: differentiation from pluripotent stem cells and epithelial organoids cultured from patient tissues. These approaches have enabled the generation of advanced human liver models and, more importantly, the establishment of patient-tailored models for evaluating individual-specific disease phenotypes and therapeutic responses. |
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