원저 : 간세포암과 만성 바이러스성 간염 환자의 간 조직 내의 Transforming Growth Factor Alpha mRNA 의 발현 ( Expression of Transforming Growth Factor Alpha mRNA in Livers of Patients with Chronic Viral Hepatitis and Hepatocellular Carcinoma ) |
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Expression of Transforming Growth Factor Alpha mRNA in Livers of Patients with Chronic Viral Hepatitis and Hepatocellular Carcinoma |
Young-Hwa Chung, M.D. Jung A Kim, M.S., Byung-Cheol Song, M.D.,
Geun Chan Lee, M.D., Moon Soo Koh, M.D., Yung Sang Lee, M.D.,
Seung Gyu Lee, M.D.,* Dong Jin Suh, M.D. |
Department of Internal Medicine and General Surgery University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea |
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ABSTRACT |
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Background/Aims : Transforming growth
factor alpha (TGFα) is an important autocrine
growth factor of hepatocytes. We were to
evaluate the roles of TGF α in chronic viral
hepatitis (CVH) and hepatocellular carcinoma
(HCC). Me thods : We measured the amounts of
TGFα mRNA in liver tissues from 18 patients
with HCC, 31 with CVH and 7 normal controls.
‘Hot start’ reverse transcription polymerase
chain reaction (RT - PCR) us ing oligo- dT and
specific primers detected TGFα mRNA in total
cellular RNA extracted from liver tissues. T he
levels of TGFα mRNA were determined by the
end point titers of serial two- fold dilutions of
cDNA. T he amounts of HBV- RNA in livers of
patients with chronic hepatitis B were also
measured by Northern blot hybridization.
Re s ults : TGFα mRNA levels were significantly
higher in HCC than CVH and normal controls ;
those of CVH were also elevated compared to
normal controls . They were overexpressed in
the nontumorous livers surrounding HCC
compared to CVH, although lower than HCC
tissues. T hey were higher in chronic hepatitis B
than in chronic hepatitis C. T hey were not
correlated with serum alanine aminotransferase
(ALT ) or HBV- RNA levels in liver tissues (in
cases of chronic hepatitis B). However, the
expression of TGFα mRNA tended to be
higher in the livers of patients with serum AFP
levels raised. Conclus ion: Overexpress ion of
TGFα mRNA in liver seems to be associated with the regeneration of hepatocytes rather than
hepatic necrosis or viral replication. Also, it
may be closely related to the development or
growth of HCC, especially in the livers of chronic
hepatitis B. |
KeyWords:
Neoplasm/ Liver / Hepatocellular carcinoma, Chronic hepatitis/Viral, Transforming growth factor alpha. |
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