| 원저 : 경쟁적 역전사-중합효소연쇄반응과 DNA-ELISA법을 이용한 C형 간염 바이러스 RNA 정량 ( Quantitation of Hepatitis C Virus RNA by Competitive RT-PCR and DNA-ELISA Method ) |
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| Quantitation of Hepatitis C Virus RNA by Competitive RT-PCR and DNA-ELISA Method |
| Kang Seok Seo, M.D., Seung Jung Kee1, M.D.,
Soon Pal Suh1, M.D., Sei Jong Kim, M.D. |
| Department of Internal Medicine and Clinical Pathology, Chonnam University Medical School, Kwangju, Korea |
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| ABSTRACT |
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Background/Aims
Quantitation of Hepatitis C Virus (HCV) RNA in serum is important for monitoring the response to interferon-α therapy in patients with chronic hepatitis C. Several commercial assays are recently available, but they are expensive and cannot be used as a gold standard. Methods: An in-house competitive reverse transcription-polymerase chain reaction (cRT-PCR) was developed and validated. The procedure involves the construction of a mutant and wild type HCV RNA internal standard (IS), cRT-PCR, and colorimetric detection with DNA-ELISA. A standard curve was obtained and used for final HCV RNA quantitation. Results: The standard curve was linear over the range of 1×104 to 5×107 copies/mL of the HCV RNA standard (r=0.976). This in-house cRT-PCR was comparable with the branched DNA (bDNA) assay (Quantiplex HCV 2.0, Chiron, USA) with positive correlation between the two tests (r=0.735). Conclusion: The quantitation of HCV RNA by in-house cRT-PCR and DNA ELISA was more sensitive and had wider range of detection compared to bDNA assay. This assay is useful for follow-up of HCV RNA concentration after interferon-α therapy.(Korean J Hepatol 2000;6:156-171) |
| KeyWords:
Hepatitis/Viral/Hepatitis C, Quantitation of Hepatitis C Virus RNA |
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