| 원저 : 알코올과 CCl4 동시투여에 의한 간경변 유도와 저용량 LPS 의 간손상 보호효과 ( Original Articles : An Experimental Model of Hepatic Fibrosis Induced by Alcohol and CCl4 : Can the Lipopolysaccharide Prevent Liver Injury Induced by Alcohol and CCl4 ? ) |
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| An Experimental Model of Hepatic Fibrosis Induced by Alcohol and CCl4 : Can the Lipopolysaccharide Prevent Liver Injury Induced by Alcohol and CCl4 ? |
| Hee Bok Chae, M.D., Lee Chan Jang, M.D.*, Seon Mee Park, M.D.,
Bo Ra Son, M.D.†, Rohyun Sung, M.D.‡, Jae Woon Choi, M.D.* |
| Department of Internal Medicine, General Surgery*, Clinical Pathology†, and Pathology‡,
College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Korea. |
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| ABSTRACT |
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Background/Aims
It is well known that alcohol enhances the toxicity of CCL4. We tried to establish an
alcoholic liver cirrhosis model by administration of alcohol and CCL4 to rats. We also wanted to know the
hepatoprotective effect of low doses of lipopolysaccharide(LPS) in this animal model. Methods: Of 20 female
adult rats, 8 were ingested with alcohol ad libitum(group 1) Another 6 were ingested with 10% alcohol and 50%
1mL/kg CCL4 intragastrically by Sonde twice a week(group 2) The remaining 6 were ingested with 10% alcohol,
CCL4, and 0.1mg/kg LPS intraperitoneally twice a week(group 3) The fibrosis was evaluated semiquantitatively
on a scale of 0(none) to 3(cirrhosis). Results: 1) After 10 weks, septal fibrosis or cirrhosis was produced in
9 out of 12 rats in groups 2 and 3 but there was no fibrotic change in group 1. 2) There was no significant
difference in pathological grading between groups 2 and 3. Conclusions: Hepatic fibrosis or cirrhosis can be
sufficiently induced by alcohol and repetitive CCL4 ingestion for 10 weeks. We can not prove the
hepatoprotective effect of low dose LPS by semiquantitative evaluation of pathological grading.(Korean J
Hepatol 2002;8:173-178) |
| KeyWords:
Liver fibrosis, Alcohol and CCl4, Lipopolysaccharide |
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