Clinical Usefulness of PIVKA-II and Lens Culinaris Agglutinin-A Reactive Alpha-fetoprotein(AFP-L3) for the Diagnosis of Hepatocellular Carcinoma

Korean J Hepatol > Volume 6(2); 2000 > Article

The Korean Journal of Hepatology 2000;6(2): 205-214.

원저 : 원발성 간암에서 PIVKA-II 및 Lens Culinaris Agglutinin-A 반응성 Alpha-fetoprotein(AFP-L3)의 임상적 유용성 ( Clinical Usefulness of PIVKA-II and Lens Culinaris Agglutinin-A Reactive Alpha-fetoprotein(AFP-L3) for the Diagnosis of Hepatocellular Carci

Clinical Usefulness of PIVKA-II and Lens Culinaris Agglutinin-A Reactive Alpha-fetoprotein(AFP-L3) for the Diagnosis of Hepatocellular Carcinoma

B.H. Byun, Y.M. Park, S.H. Bae, J.Y. Choi, K.Y. Sung, S.H. Cho, N.I. Hahn, J.M. Yang, S.O. Choi, S.B. Cha, K.W. Chung, H.S. Sun, D.H. Park, B.S. Kim

Department of Internal Medicine Catholic University of Korea, College of Medicine, Seoul, Korea

ABSTRACT

Background/Aims
Des-γ-carboxy prothrombin(DCP), a protein induced by vitamin K absence or antagonist-II(PIVKA-II), and Lens culinaris agglutinin-A reactive AFP-L3 have been reported to be useful markers for the diagnosis of hepatocellular carcinoma (HCC). In the present study, both the PIVKA-II and AFP-L3 were analyzed and compared with a conventional AFP to determine its usefulness, specificity and sensitivity in the diagnosis of HCC. Methods: Sera were collected from 108 patients consisting of 17 patients with chronic hepatitis, 22 patients with liver cirrhosis and 69 patients with HCC. The AFP-L3 was determined by an lectin affinity electrophoresis coupled with an antibody-affinity blotting. Level of DCP was measured by an enzyme immunoassay with an anti-DCP monoclonal antibody. Results: The sensitivity and specificity of PIVKA-II and AFP L3 were 49.3% and 89.5%, and 32.5% and 85.7%, respectively. No significant correlation was found between the PIVKA-II or AFP L3 and serum AFP. No correlation was found between the PIVKA-II or AFP L3 and the characteristics of HCC. Conclusion: The determination of plasma DCP and AFP L3 levels combined with AFP levels may be useful especially for the differential diagnosis between HCC and chronic liver diseases without HCC.(Korean J Hepatol 2000;6:205-214)

KeyWords: Neoplasm/Liver/Hepatocellular Carcinoma, Alpha- fetoprotein, AFP-L3, PIVKA- II, Des-γ- carboxy prothrombin(DCP), Tumor markers